People

Prof., RNDr. Pavol Miškovský, PhD., DrSc.

Head of the Center for Interdisciplinary Biosciences, P.J. Safarik University, Kosice
CEO SAFTRA Photonics LtD
Specialist in biophotonics. PhD degree in Biophysics (1986), Charles University, Prague, D.Sc. degree in Biophysics (2000), Comenius University Bratislava, full professor in Physics (2000). Director of Master and Doctoral study in Biophysics, UPJS, Kosice, Slovakia. President of the committee for D.Sc. in Biophysics in Slovak Republic.

Education:

1978 M.Sc. in Physics, Charles University, Prague, Czech Republic
1979 Rerum Naturalium Doctor in Physics, Charles University, Prague, Czech Republic
1986 Ph.D. in Biophysics, Charles University, Prague, Czech Republic
1993 Associate Professor (habilitation), Comenius University, Bratislava, Slovakia
2000 Doctor Scientarium (D.Sc.) in Biophysics, Comenius University, Bratislava, Slovakia
2001 Full Professor in Physics, P. J. Safarik University, Kosice, Slovakia

Languages

fluent in English, French, Russian, Czech and Slovak

Positions:

•Head of the Center of Interdisciplinary Biosciences, P. J. Safarik University, Kosice, Slovakia

  • CEO SAFTRA Photonics Ltd

•Full Professor and guarantee of undergraduate and doctoral study in biophysics, P.J. Safarik University, Kosice, Slovakia
•Senior researcher, International Laser Center, Bratislava, Slovakia (0,3 duty)

Postdoctoral research training:

•1987–1988 Institute Curie, Lab. de Physicochimie Biomol. et Cellulaire, Paris, France (6 months)
•1992 Institute Curie, Lab. de Physicochimie Biomol. et Cellulaire, Paris, France (11 months)
•1993 Dipartimento di Biochimica, Universitá di Bologna, Italy, (2 months)

Invited professor positions:

• 1993–1996, Université P. et M. Curie, Paris, France, (4 month/year)
• 1998, Université P. et M. Curie, Paris, France, (1 month)
• 2003, Université d’Orleans, Orleans, France (1 month)
• 2004, Instituto de Estructura de la Materia, CSIC, Madrid (3 months sabbatical stay)
• 2005–2007, Université d’Orleans, Orleans, France (2 months/year)
• 2006 Iowa State University, Ames, USA (3 months) – Fulbright fellowship
• 2011 Université P. et M. Curie, Paris, France, (1 month)
NATO “Expert visit” grants
• Instituto de Estructura de la Materia, Madrid, Spain, 1997, 2003 (three weeks/year)
• Université P. et M. Curie, Paris, France, 2000 (three weeks)

Honor and awards

•Award of the Czechoslovak government for results in applied science 1989
•Awards from international fairs: Gold medal for „Flow Injection Analyzer PIA-O1“ from international fair „Zeme Zivitelka“ – Ceské Budejovice, Czech Republic, 1989
•Award of the President of the Faculty of Sciences, Safarik Univ. (results in science), 1997, 2010
•invitations for prestigious publications peer reviewed journals (see publications of P.M.)
•qualification for “Full Professor” positions in France (2002–2005) – branches: 1) 28 – condense matter and materials, and 2) 30 – diluted matter and optics
•member of the Int. Scientific and Advisory Committee of the Seventh and the Eighth Int. Conf. of Solar En. Storage and Appl. Photochrm. (2003 & 2005 Luxor, Egypt)

Research interest:

P.M. is working in interdisciplinary bio-research, recently mainly oriented to (see research grants):

  1. photo-physics, photo-chemistry and photo-biology of antitumor photoactive agents: mechanism of their antitumor action (from molecular and cellular levels up to application in photodynamic therapy of cancer)

ii) structure and conformation of biological macromolecules and their interactions with drugs (application of sophisticated biophotonics methods)

Selected scientific projects (P.M. principal investigator):

I. Science grant agency of the Ministry of education of the SR and of the Slovak Academy of Sciences (VEGA)

1.VEGA 1/3258/96 (1996 – 1998) Antiviral activity of aromatic polycyclic dions: Mechanism of interaction on cellular and molecular levels: 10 000 €, principal investigator
2.VEGA 1/6027/99 (1999–2001) Antiviral and Antitumor Activity of Hypericin and Hypocrellin: Mechanism of inter. at cell. and mol. levels and its correlation with intramol. proton transfer: 12 000 €, principal investigator
3.VEGA 1/9040/02 (2002 – 2004) Antiviral and Antitumor Activity of nat. photoactive mol. Hypericin and Hypocrellin: Int. with boil. Macromol. and mechanism of action at cell. and mol. levels: 11 000 €, principal investigator
4.VEGA 1/2278/05 (2005 – 2007) Interaction of natural photosensitizers hypericin and canphostin C with regulatory and transport proteins: 15 000 €, principal investigator

II. Science and Technology Assistance Agency (APVV)

5.APVT-20–0222–02 (2002–2004) New Physical and Chemical Approaches to Photodynamic Therapy of Cancer: 98000 €, principal investigator
6.APVV-20–036104 (2005–2007) Photodynamic Therapy of Cancer : Mechanism of action of new antrachinones molecules: 135 700 €, principal investigator
7.APVV-0449–07 (2008–20010) Targeted photodynamic therapy> 280 000 €, principal investigator
8.APVV- LPP 0337–06 (2006–2010) Photodynamic therapy of cancer in the light of new molecular knowledge: 139.000 €, principal investigator
9.APVV- LPP 0072–07(2008–2012) Increasing of the efficiency of cancer therapy by targeted transport of photoactive drugs: 158.000 €, principal investigator
10.APVV- LPP 0290–09 (2009–2013) Development of optical nanosensors for multicomponent analysis of trace quantities of drugs and environment pollutants: 83.000 €, principal investigator

III. Grants of the European Union:

11.TEMPUS JEP 2327, (1991–1994) Centers for Master’s and Doctoral Studies, and Continuous Education in Biophysics and Chemical Physics: ~150 000 USD, for the Department, co-PI, responsibility for the Slovak partner)

12.Marie Curie reintegration grant No PIRG06-GA-2009–256580 (2010–1013): “MonInterFluoProt” 75.000 €, principal investigator

13.FP7 (2013–2016) “Fostering excellence in multiscale cell imaging” CELIM 316312, 2.700.000 €, principal investigator

14. H2020 (2016) “Disruptive portable device for pre-screening of Persistent Organic Pollutants –POPs- in food products and water (NanoScreen 66266)” SME instrument NMP 25 – 2014/2015, principal investigator

IV. Grant agencies of the USA:

14. NSF-CHE 1396 (1998–2001), Proton Transfer in Biomolecules: Interaction of Hypericin with Model Cellular Components: 40.000 USD, for the Department, coPI

V. NATO grants:

15. CBP.EAP.CLG 981432 (2005–2007), Detection of trace quantities of molecules by chemical sensors based on metallic nano-particle surfaces: 12.500 € principal investigator

VI. Internacional bilateral projects:

16.France-Slovakia, (1998–2001) Development and Characterization of a New Class of Antiviral and Antitumor Photoactive Agents: 9.000 € for the Slovak partner coPI

17. France-Slovakia, (project Stefanik) (2006–2007) Intracellular mechanism of anticancer activity of photoactive molecules: 4.000 €, for the Slovak partner, coPI

18.Czech Republic – Slovakia, 343/300, (2000–2002) Study of anticancer and antiviral activity of polycycling dions: 3.000 € for the Slovak partner, coPI

19. Czech Republic – Slovakia, 021/042 (2003–2005), Study of anticancer and antviral activity of new photoactive agents hypericin and hypocrellinA: 5.000 € for the Slovak partner, coPI

VII. Structural Regional Grants of the European Union:

20. SEPO (Network of excellence in oncology) (2009–2011): 120.000 € for the Department, coPI
21. CEVA (Center of excellence for respiration diseases) (2010–2012): 750.000 € for the Department, coPI
22. NanoBioSens (Development of optical nano-sensors for multicomponent analysis of trace quantities of pollutants and drugs) (2011–2013): 800.000 € for the Department, principal investigator
23. DOKTORAND (Modernization of doctoral study in natural sciences) (2010–2013): 250.000 € for the Department, principal investigator

Experience with EU FP6, FP7 and H2020 projects:

yes (FP6 (coPI), FP7(coPI) and H2020 (PI), EU expert (PF5–7, H2020))

Management activities

P.M. is
•guarantee of undergraduate and doctoral study in Biophysics at P. J. Safarik Univ., Kosice, Slovakia
•president of the habilitation (nomination of docents) and inauguration (nomination of professors) commission in biophysics at P.J. Safarik University, Kosice, Slovakia
•president of the National Scientific Committee for D.Sc. ( Doctor Scientarium ) degree in biophysics
•member of the Science Committee of the Faculty of Sciences, Safarik Univ., Kosice, Slovakia
•member of the Executive Committee of the European Biophysical Societies' Association (EBSA) 2013 -
•member of the Executive Committee of the Slovak Biophysical Society (SKBS) 2009 -
•president of the Slovak Bio-Imaging Network 2013 – (member of the Euro-BioImaging Network)
•Memer of the the interim board of the pan-European infrastructure network Eurro-BioImaging 2013 –

P.M.was
•member of the Comm. for doctoral thesis defense, P. et M. Curie Univ., Paris, France, 1995, 1998, 2000
•member of the Habilitation Committee, Orleans Univ., Orleans, France (2004)
•president of the organizing committee of the symposium of the Slovak biophysical Society 2004, 2006
•member of the Committee for Doctoral Study in Physics of Molecular and Biological Structures, Charles University, Prague, Czech Republic, 1992–2000
•member of the National Committee for doctoral study in Biophysics, Faculty of Mathematics and Physics, Comenius University, Bratislava, 1991 – 1996, and vice-president 1997-
•member of the Steering Committee and national coordinator of the EU JEP 2327 TEMPUS project (1991–94)
•responsible for the agreement between the Safarik Univ. and the Univ. P. et M. Curie, Paris, France (from 1994)
•member of the group of experts of the Slovak Accreditation Commission (Physics) (1998–2002)
•member of the Science Committee of the Fac. of Medicine, P.J.Safarik Univ., Kosice, Slovak Republic (1998–2004)
•chairman of the international Workshop on Biophotonics and Molecular Simulations, Bratislava 2006
•president of the Slovak Biophysical Society, 2001–2009

International collaboration of P.M.:

University of P. et M. Curie, Paris, France, Dep. of Chemistry, Iowa State University, USA, Instituto de Estructura de la Materia, Madrid, Spain, Institute of Physics, Charles University, Prague, Czech Republic, Swiss Federal Institute of Technology, Switzerland , Synchrotron SOLEIL, Gif-sur Yvette, France, Centre de Biophysique Moléculaire, CNRS, Orleans, France,

Invited congress lectures:

41 lectures (international congresses) including 4 plenary lectures

Other invited lectures:

46 invited lectures at Universities and Research canters, in 6 countries (USA, France, Spain, Italy, Czech Republic and India)

Graduated PhD students:

•Alena Ansary (thesis defense: Université P. et M. Curie, Paris 1995)
•Jozef Ulicny (thesis defense: University of P. J. Safarik, Kosice 1996)
•Branislav Brutovsky (thesis defense : University of P. J. Safarik, Kosice 1998)
•Daniel Jancura (thesis defense: University of Comenius Bratislava 1999)
•Gabriela Fabriciova (thesis defense University of Comenius, Bratislava 1999)
•Eva Kocisová (thesis defense: Université P. et M. Curie, Paríž 1995) – co-tutoring doctoral study, with Paris VI University, France
•Jana Stanicova (thesis defense: University of Comenius, Bratislava 2001)
•Jozef Hitz (thesis defense: University of Comenius, Bratislava 2005)
•Silvia Kocanova (thesis defense : University of P. J. Safarik, Kosice 2005)
•Slávka Kascakova, (thesis defense: University of P. J. Safarik, Kosice 2005) – co-tutoring doctoral study, with University of Orleans, France
•Peter Gbur , (thesis defense: University of P. J. Safarik, Kosice 2010)
•Gejza Lajos , (thesis defense: University of P. J. Safarik, Kosice 2010)
•Stefan Balint, ( thesis defense: University of P. J. Safarik, Kosice 2010)
•Veronika Huntosova, (thesis defense: University of P. J. Safarik, Kosice 2010) – co-tutoring doctoral study, with Paris VI University
•Luboslava Buriankova, (thesis defense: University of P. J. Safarik, Košice 2010)
•Lenka Bryndzova, (thesis defense: University of P. J. Safarik, Košice 2012)
•Dana Petrovajova, (thesis defense: University of P. J. Safarik, Košice 2013)
•Diana Buzova, (thesis defense: University of P. J. Safarik, Košice 2011)
•Alena Strejčková (thesis defense: University of P. J. Safarik, Košice 2013)

Publications:

124 publications in international peer-reviewed journals (see WOS database), cited more than 1000 times, H index 22

Kosice, October, 2016

×

Assoc. prof. Gregor Bánó, PhD.

Gregor Bánó received an M.S. degree in 1997 at Charles Univ. in Prague, Czech Rep. (physical electronics and plasma physics) and a PhD in 2003 at Univ. of Szeged, Hungary (optics and opto-electronics). He held research position at the Research Institute for Solid State Physics and Optics in Budapest (Hungary) from 2001–2006, and joined the Department of Biophysics at P.J.Šafárik Univ. in Košice in 2007. His present research interest is focused on the experimental investigation of drug transport phenomena by means of different laser spectroscopic techniques.

Stages abroad

1996 (3 months) University of Innsbruck, Austria
1999–2002 (8 months) JILA Boulder, USA
2002–2003 (6 months) Charles University, Prague, Czech Rep.
2005 and 2007 (6 months) University of Colorado, Boulder, USA

Publications

  1. J. Glosík, G. Bánó, E.E. Ferguson, W. Lindinger: Selected ion flow drift tube study of the formation and dissociation of CO+.N2 ions in nitrogen buffer gas: the CO+.N2 bond energy, Int. J. Mass Spectr. 176, 177–188 (1998)
  2. J. Glosík, G. Bánó, R. Plašil, A. Luca, P. Zakouril: Study of the electron ion recombination in high pressure flowing afterglow: recombination of NH4.(NH3)2, Int. J. Mass Spectr. 189, 103–113 (1999)
  3. Z. Donkó, G. Bánó, L. Szalai, K. Kutasi, K. Rózsa, M. Pinheiro, N. Pinhao: Investigations on the effect of constriction in the cathode region of argon glow discharges, J. Phys. D: Appl. Phys. 32, 2416–2425 (1999)
  4. G. Bánó, L. Szalai, K. Kutasi, P. Hartmann, Z. Donkó, K. Rózsa, Á. Kiss, T. M. Adamowicz: Operation characteristics of the Au-II 690-nm laser transition in a segmented hollow-cathode discharge, Appl. Phys. B. 70, 521–525 (2000)
  5. P. Hartmann, Z. Donkó, G. Bánó, L. Szalai, and K. Rózsa: Effect of different elementary processes on the breakdown in low-pressure helium gas, Plasma Sources Science and Technology 9, 183–190 (2000)
  6. G. Bánó, P. Horváth, K. Rózsa: Cathaphoretic confinement of Zinc evaporated into helium and neon discharges, J. Phys. D: Appl. Phys. 33, 2611–2617 (2000)
  7. A. Bogaerts, Z. Donkó, K, Kutasi, G. Bánó, N. Pinhao, M. Pinheiro: Comparison of calculated and measured optical emission intensities in a direct current argon-copper glow discharge, Spectrochimica Acta B. 55, 1465–1479 (2000)
  8. G. Bánó, A. Luca, J. Glosík, P. Zakouřil, W. Lindinger: Formation of SiH3+ ions in reactions of small hydrocarbon ions with SiH4, Czech. J. Phys., 50, Suppl. S3, 251–257, (2000)
  9. K. Rózsa, G. Bánó, A. Gallagher: The location of very small particles in silan RF discharge, IEEE Trans. on Plasma Sci. 29, 256–260 (2001)
  10. P. Macko, R. Plašil, P. Kudrna, P. Hlavenka, V. Poteyra, A. Pysanenko, G. Bánó, J. Glosík: High-resolution cw-diode laser cavity ring-down spectroscopy in hydrogen plasma at room temperature, Czech. J. Phys. 52, Suppl. D, D695-D704 (2002)
  11. G. Bánó, L. Szalai, P. Horváth, K. Kutasi, Z. Donkó, K. Rózsa, T. M. Adamowicz: Au-II 282 nm segmented hollow cathode laser – parametric studies and self-consistent modeling; J. Appl. Phys. 92, 6372 (2002)
  12. A. Gallagher, G. Bánó, K. Rózsa: Particles in silicon deposition discharges, Solar Energy Materials & Solar Cells 78, 27–40 (2003)
  13. G. Bánó, P. Horváth, Z. Donkó, K. Rózsa, T. M. Adamowicz: Sputtered and heated high-voltage hollow-cathode zinc lasers, Appl. Phys. B. 77, 403–407 (2003)
  14. P. Macko, G. Bánó, P. Hlavenka, R. Plasil, V. Poterya, A. Pysanenko, O. Votava, R. Johnsen, J. Glosík: Afterglow studies of H3+(v=0) recombination using time resolved cw-diode laser cavity ring-down spectroscopy, Int. J. Mass Spectr. 233, 299–304 (2004)
  15. G. Bánó, P. Horváth, L. Csillag, J. Glosík, T. M. Adamowicz, K. Rózsa: 224 nm segmented hollow-cathode silver ion laser, Appl. Phys. B. 80, 215–219 (2005)
  16. K. Kutasi, P. Hartmann, G. Bánó, Z. Donkó: He2+ molecular ions in helium glow discharges: the effect of bulk electron temperature, Plasma Sources Sci. Technol. 14, S1-S8 (2005)
  17. G. Bánó, P. Horváth, K. Rózsa, A. Gallagher, The role of higher silanes in silane-discharge particle growth, J. Appl. Phys. 98, 013304 (2005)
  18. P. Hlavenka, R. Plašil, G. Bánó, I. Korolov, D. Gerlich, J. Ramanlal, J. Tennyson and J. Glosík: Near infrared second overtone cw-cavity ringdown spectroscopy of D2H+ ions, Int. J. Mass Spectr. 255–256, 170–176 (2006)
  19. Z. Sternovsky, K. Amyx, G. Bano, M. Landgraf, M. Horanyi, S. Knappmiller, S. Robertson, E. Grün, R. Srama, S. Auer: Large area mass analyzer instrument for the chemical analysis of interstellar dust particles, Rev. Sci. Instrum. 78, 014501 (2007)
  20. G. Bánó, P. Hartmann, K. Kutasi, P. Horváth, R. Plašil, P. Hlavenka, J. Glosík, Z. Donkó: Combined Langmuir probe, electrical and hybrid modelling characterization of helium glow discharges, Plasma Sources Sci. Technol. 16, 492–500 (2007)
  21. G. Bánó, P. Horváth, T. M. Adamowicz, K. Rózsa: A novel design and operation scheme for a 224 nm segmented hollow-cathode silver ion laser, Appl. Phys. B. 89, 333–337 (2007)
  22. A. Derzsi, P. Hartmann, I. Korolov, J. Karácsony, G. Bánó, Z. Donkó: On the accuracy and limitations of fluid models of the cathode region of dc glow discharges, J. Phys. D: Appl. Phys. 42, 225204 (2009)
  23. R. Plašil, I. Korolov, T. Kotrik, P. Dohna, G. Bánó, Z. Donkó, J. Glosík: Non-Maxwellian electron energy distribution function in He, He/Ar, He/Xe/H-2 and He/Xe/D-2 low temperature afterglow plasma, Eur. Phys. J. D. 54, 391–398 (2009)
  24. I. Korolov, G. Bánó, Z. Donkó, A. Derzsi, P. Hartmann: Experimental study of the asymmetric charge transfer reaction between Ar+ ions and Fe atoms, J. Chem. Phys. 134, 064308 (2011)
  25. G. Bánó, J. Staničová, D. Jancura, J. Marek, M. Bánó, J. Uličný, A. Strejčková, P. Miškovský: On the diffusion of hypericin in dimethylsulfoxide/water mixtures – the effect of aggregation, J. Phys. Chem. B. 115, 2417–2423 (2011)
  26. I. Korolov, G. Bánó, Z. Donkó: Investigation of the asymmetric charge exchange reaction in the Ar±Ni system in the afterglow of a pulsed glow discharge, Spectrochim. Acta B. 66, 706–7011 (2011)
  27. G. Bánó, Z. Donkó: On the high argon metastable atom density measured near the cathode surface of a hollow cathode discharge, Plasma Sources Sci. Technol. 21, 035011 (2012)
  28. A. Strejčková, J. Staničová, D. Jancura, P. Miškovský, and G. Bánó: Spatial orientation and electric-field-driven transport of Hypericin inside of bilayer lipid membranes, J. Phys. Chem. B. 117, 1280–1286 (2013)
  29. M. A. Omar, P. Miskovsky, G. Bánó: Proof-of-principle for simple microshelter-assisted buffer exchange in laser tweezers: interaction of Hypericin with single cells, Lab Chip 14, 1579–1584 (2014)
  30. J. Varchola,V. Huntosova, D. Jancura, G. Wagnieres, P. Miskovsky, G. Bánó: Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation, Photochem. Photobiol. Sci. 13, 1781–1787 (2014)
  31. G. Vizsnyiczai, T. Lestyán, J. Joniova, B. L. Aekbote, A. Strejčková, P. Ormos, P. Miskovsky, L. Kelemen, G. Bánó: Optically Trapped Surface-Enhanced Raman Probes Prepared by Silver Photoreduction to 3D microstructures, Langmuir 31, 10087−10093 (2015)
  32. J. Varchola, K. Želonková, D. Chorvat Jr, D. Jancura, P. Miskovsky, G. Bánó: Singlet oxygen produced by quasi-continuous photo-excitation of hypericin in dimethyl-sulfoxide, J Lumin 177, 17–21 (2016)
  33. J. Joniova, M. Rebič, A. Strejčková, V. Huntosova, J. Staničová, D. Jancura, P. Miskovsky, G. Bánó: Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles – Experiments and Modeling, Biophys. J. 112, 966–975 (2017)
  34. A. Hovan, S. Datta, S. G. Kruglik, D. Jancura, P. Miskovsky, G. Bánó: Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part I. Theory, J. Phys. Chem. B 122, 5147–5153 (2018)
  35. S. Datta, A. Hovan, A. Jutková, S. G. Kruglik, D. Jancura, P. Miskovsky, G. Bánó: Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The case of Hypericin-loaded Low-Density Lipoprotein Particles, J. Phys. Chem. B 122, 5154–5160 (2018)
×

RNDr. Marián Fabián, CSc.

Senior research associate in the Center for Interdisciplinary Biosciences, Faculty of Natural Sciences, Safarik University in Kosice (UPJS)
Specialist in enzymology of the respiratory heme-copper oxidases, heme proteins, the primary reactions in photosynthesis and the bacterial iron acquisition systems

Education:
1964 – 67 High school, Handlova, Czechoslovakia
1969 – 74 Charles University, Prague, Czechoslovakia, – MSc. in Biophysics
1977 – 81 Moscow State University, Moscow, USSR – CsC. degree in Biophysics

Languages
Slovak, Russian and English

Professional positions:
2013 – present Senior Research Associate at Centre for Interdisciplinary Biosciences, P.J. Šafárik University, Košice, Slovak Republic
1998 – 2013 Faculty and Senior Faculty Fellow, Department of Biochemistry and Cell Biology, Rice University, Houston
1984 – 1991 Head of Biophysical Laboratory, Institute of Experimental Physics, Slovak Academy of Sciences, Kosice, Czechoslovakia

1981 – 1984 Head of Biophysical Laboratory, Neurobiological Institute of Slovak Academy of Sciences, Kosice, Czechoslovakia

1974 – 1977 Research Fellow, Neurobiological Institute of Slovak Academy of Sciences, Kosice, Czechoslovakia.

Postdoctoral research training:
1991 – 1998 Postdoctoral Research Associate at Department of Biochemistry and Cell Biology, Rice University, Houston with Prof. G. Palmer

Honors
Award of Slovak Biophysical Society for the substantial contribution to the development of Biophysics in Slovakia, 2016.

Awards
2009–2011 NIH grant: Molecular mechanism of redox driven proton pumps Role: Principal Investigator

2002 – 2006 NIH grant: Catalytic Mechanism of Heme-Copper Oxidases Role: Principal Investigator

1998 – 2002 Co-principal investigator in NIH grant: Catalytic Mechanism of Heme-Copper Oxidases

1991 – 1993 Grant from Slovak Academy of Sciences, Czechoslovakia. Role: Principal Investigator

1986 Short term grant from Swedish Academy of Engineering Sciences

Technical expertise
Experience with variety of spectroscopic methods (optical spectroscopy, electron paramagnetic resonance (EPR), magnetic circular dichroism, Fourier-Transform infrared spectroscopy), fast kinetic methods (stopped flow, laser flash photolysis, rapid-freeze quench method) and methods employed in purification of proteins and computer analysis of data.

Publications:
49 publications in international peer-reviewed journals (see WOS and Scopus databases), cited more than 800 times, Hirsch index ≈19
Kosice, November 2016

×

doc. RNDr. Denis Horváth, PhD.

EDUCATION

MSc. Solid-state physics, Dept. of Experimental physics, Faculty of Science,
UPJŠ, Košice, Slovakia

PhD. (CSc.) Solid-state physics, Dept. of Magnetism, Institute of Experimental Physics,
Slovak Academy of Sciences, Košice, Slovakia

EMPLOYMENT, STAGES, SCIENTIFIC CAREER :

1989–1997 Institute of Experimental Physics, Slovak Academy of Sciences, scientific fellow, senior scientist;

  • stage (1/2 year) Bogoliubov Laboratory of Theoretical Physics, Joint Institute for Nuclear Research, Dubna, Russian Federation;
  • stage Dept. Physics, Helsinky University, Finland;

1997–2002 Faculty of Natural Science, Šafárik University, Košice, Slovakia, associated professor, lecturing experience;

  • stage Dept. of Solid State Physics, University of Lodz, Poland;
  • stage International Centre for Theoretical Physics, Trieste, Italy;

2008–2009 stage „le STUDIUM“ institute for advanced studies (International research center and hosting foreign associate researchers, organized into a network of European laboratories, based on the Région Centre), CBM (Center de Biophysique Moleculaire Orleans France) CNRS (Centre National de la Recherche Scientifique), group of Theoretical Biophysics, Molecular Simulation, and Scientific Computing;

2009–2011 SORS research joint stock company, Košice, Slovakia, data scientist and analyst;

2011–2012 Technical University of Košice, Faculty of Economics, Dept. Finance, position of scientist and lecturer;

2012–2015 Center of Interdisciplinary bioscience (CIB), UPJŠ Košice, Slovakia

Research interests, expertise relevant to the project:

  • continuous models, newtonian and non-newtonian complex fluids
  • theory of randomly stirred incompressible fluids
  • renormalization group field-theoretical approaches
  • computational and statistical physics
  • discrete media models in solid state (condensed matter) physics
  • mean field theoretical methodologies
  • Monte Carlo simulation technique, adaptive Monte Carlo schemes
  • molecular dynamics technique
  • nonlinear ordinary differential equations solvers and sensitivity analysis for time-dependent systems
  • coarse-grained and multi-scale computational models, nanostructures
  • time-series data statistical analysis, autoregressive models
  • clustering algorithms, distance-based methodologies
  • agent-based and multi-agent simulations
  • neural networks and related tools of artificial intelligence, information processing of system configurations and reconstructed experimental images
  • continuous-time deterministic models based on the ordinary differential equations
  • large-scale models of entropy-controlled hypercycles and biological structures
  • computational models of the growth dynamics of multi-cellular tumor spheroids
  • presently: acquiring theoretical background for biostatistics and sensitivity analysis,

Computational skills:
Linux operation system, elementary Bash-Scripting
C and C++ programming skills
R programming and statistical computing
Mathematica – advanced symbolic computing
Condor distributed computing practice

List of selected publications related to the project specifications and requirements:

topic: renormalization group, models of fluids
Buša J., Hnatich M., Honkonen J., Horváth D.: “Stability of Kolmogorov scaling in anisotropically forced turbulence”, Phys. Rev. E, 55 (1997) 381–394.

topic: molecular dynamics simulation technique
Kašpárková M., Horváth D., “On positional ordering in a 2-dimensional system of parallel dipoles”, J. Phys.D: Appl.Phys. 25 (1992) 1522–1527.

topic: Monte Carlo, tuning of simulation outputs to experiment
Horváth D., Orendáčová A., Orendáč M., Jaščur M., Brutovský B., Feher A.: “Ker(MoO4)_2: A quasi one-dimensional S=3/2 Blume-Capel system”, Phys. Rev. B 60 (1999) 1167–1174.

topic: neural networks, adaptivity and artificial intelligence in nanostructures and solid state physics
Horváth D., Gmitra M., Vávra I.: “Neural network approach to magnetic dot arrays”, J. Magn. Magn. Mater. 231 (2001) 273–286.

topic: multi-scale approach, solid state physics and artificial intelligence
Horváth D., Gmitra M.:“The neural network two-scale model of the magnetic dot array”, J. Magn.Magn.Mater. 263 (2003) 195–213.

topic: inverse problem within the Monte Carlo technique
Horváth D., Gmitra M., Kuscsik Z.: “The self-organized Monte Carlo localization of the critical point via linear filtering”, Czech. J Phys 54 (2004) 921– 926.

topic: multi-scale simulations and Monte Carlo method
Horváth D., Gmitra M.: “The self-organized multi-lattice Monte Carlo simulation”, Int. J. Mod. Phys. C 15 (2004) 1249–1268.

topic: multicanonical Monte Carlo method, coarse-grained simulations
Reitzner D., Horváth D.: “Multicanonical sampling of vortex states in magnetic nanoelements”,
Physica A 379 (2007) 587–594.

topic: biological systems, chemical kinetics, simulations
Horváth D., Kneller G.R.: “A least-constraint principle for population dynamics and reaction kinetics,
Modeling entropy-controlled chemical hypercycles”, J. Chem. Phys., 131:171101, 2009.

topic: inverse problems in the simulations in the biological systems
Horváth D., Brutovský B., Kočišová J., Šprinc S.: “Manipulation with heterogeneity within a species population formulated as an inverse problem”, Physica A, 389 (2010) 5028–5036.

topic: coarse-grained models of biological systems, optimization
Brutovský B., Horváth D.: „The optimization aspects of carcinogenesis“,
Medical Hypotheses (2009), doi:10.1016/ j.mehy.2009.10.019; [see also arXiv:0907.2004v3 (q-bio.PE)].

B. Brutovsky, Horvath D. “Towards inverse modeling of intratumor heterogeneity”, Open Physics, 13 (2015) 232–241.

topic: coarse-grained models of biological systems, inverse approach
Brutovský B., Horváth D., Lisý V.: “Inverse geometric approach for the simulation of close-to-circular growth. The case of multicellular tumor spheroids”, Physica A 387 (2008) 839–850.

topic: data mining, manifold learning, advanced hybrid optimization
Horvath D., Ulicny J., Brutovsky B.: “Self-organised manifold learning and heuristic charting via adaptive metrics”, Connection Science 28 (1), (2016) 1–26.

topic- modeling of compartmental intracellular transport
Verebova V., Belej D., Joniova J. , Jurasekova Z., Miskovsky P., Kozar T., Horvath D. , Stanicova J., Huntosova V.: “Deeper insights into the drug defense of glioma cells against hydrophobic molecules”, International Journal of Pharmaceutics, 503 (1–2) (2016) 56–67.

topic – biostatistics and phenomenological modeling
Misuth M., Joniova J., Belej D., Hrivnak S., Horvath D., Huntosova V.: “Estimation of PKCδ autophosphorylation in U87 MG glioma cells: combination of experimental, conceptual and numerical approaches”, Journal of biophotonics (2016).

topic – advanced biostatistics
Huntosova V., Gerelli E., Horvath D., Wagnieres G.: “Measurement of pO2 by luminescence lifetime spectroscopy: A comparative study of the phototoxicity and sensitivity of [Ru(Phen)3]2+ and PdTCPP in vivo”, Journal of Biophotonics, online : 2 SEP 2016, DOI: 10.1002/jbio.201600127

topic – Monte Carlo simulation of evolutionary phenomena on the level of cellular populations
Horvath D., Brutovsky B.: “Etiology of phenotype switching strategy in time varying stochastic environment, Physica A: Statistical Mechanics and its Applications, 462 (2016) 455–468.

topic – Monte Carlo simulation of evolutionary phenomena on the level of cellular population
Horvath D., Brutovsky B.: “Study of selected phenotype switching strategies in time varying environment”, Physics Letters A, 380, (13) (2016) 1267–1278.

×

Dr. Veronika Huntošová, PhD.

Dr. Veronika Huntosova, PhD (34) (f), scientific researcher at the Center for Interdisciplinary Biosciences is a specialist in time-resolved spectroscopic and imaging techniques, detection of tissue oxygenation and metabolic changes, cancer photo-detection and photo-treatment. She obtained double PhD degree in Biophysics at P.J. Safarik University in Kosice, Slovakia and Dr. of P. and M. Curie University Paris 6, France (2010). She spent two years as a post-doc at internationally recognized scientific institution: Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland (2011–3). Dr. Huntosova has published 14 peer-reviewed articles (9 of them is the first author). These papers have been cited more than 60 times (excluding auto-citations). She was a principal investigator of 1 international project (Sciex-NMSch, Switzerland), principal investigator of 2 national project at University level (1 Lpp Slovak Research and Development Agency, 1 VVGS, UPJS Kosice, Slovakia), co-investigator of international projects (1 CELIM EU, 1 PICS, CNRS France, 1 Swiss National Science Foundation) and national projects (1 APVV, 1 VEGA). Dr. Huntosova has been honored by several awards: Slovak Biophysical Society award for young investigator (2016), 2 x the best oral or poster presentation at international congresses (1 CSEM, Neuchatel, Switzerland (2011), 1 International Union of Photobiology, Cordoba, Argentina (2014)), a honourable mention of the president of Faculty of Science of UPJS in Kosice (2006), Slovak literary found award for excellent student scientific work (2006), 1st place in the Biophysical and Biomedical section at the National Student Scientific Conference in Bratislava (2006), 2nd place in Biochemical section at Student Scientific Conference in Košice (2006). She also obtained grant supports from European Society for Photobiology (2× ESP) and Czech-Slovak woman foundation for young women in science.

×

Assoc. Prof. Daniel Jancura, PhD.

Associate professor at Department of Biophysics, Faculty of Natural Sciences, Safarik University in Kosice (UPJS), PhD. degree in Biophysics (1999), Comenius University in Bratislava (Slovakia), associate professor in Biophysics at UPJS from 2009. Specialist in molecular biophysics and molecular bioenergetics, recent research interests: structure and stability of biomacromolecules and their complexes with low molecular ligands, construction and characterization of lipoprotein based drug delivery systems, catalytical mechanism of cytochrome c oxidase.

Education

1976 – 84 Elementary school, ZS Hrnčiarska Humenné
1984 – 88 High school, Gymnázium Humenné
1988 – 93 Safarik University Kosice (Slovakia) – MSc. in Biophysics
1993 – 97 Safarik University Kosice – PhD study in Biophysics
1999 Comenius University Bratislava (Slovakia)- Defence of PhD thesis

Professional positions

1997 – 1999 Assistant professor, Department of Biophysics, Safarik University Kosice
2002 – 2009 Assistant professor, Department of Biophysics, Safarik University Kosice
2009 – Associate profesor, Department of Biophysics, Safarik University, Kosice

Foreign stays

1993 Dipartimento de Biochimica, Universita Bologna (Italy)
1996 – 1997 Instituto de Estructura de la Materia, CSIC , Madrid (Spain)
1999 – 2002 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2005 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2010 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2011 Department of Biochemistry & Cell biology, Rice University, Houston (USA)

Professional orientation

Optical spectroscopy (UV.VIS absorbance, fluorescence, Raman) and microcalorimetry of biomacromolecules and their complexes with biological imporatnts ligands
Construction and characterization of lipoprotein based drug delivery systems
Molecular mechanisms of photodynamic therapy
Catalytical mechanism of cytochrome c oxidase

Number of articles in CC journals : 35
Number of SCI citations : 208

Hobby

Sport (football, tenis, basketball)
Literature (scientific literature, politics, economy, Music (60‘s)

List of CC publications

  1. G. Fabriciová, P. Miškovský, D.Jancura, E. Kočišová and V. Lisý. Conformational transitions of poly ( dI-dC) as studied by classical Raman spectroscopy. Gen. Physiol. Biophys. 14, 203 216 (1995).
  2. D.Jancura, S. Sánchez-Cortés, E. Kočišová, A Tinti, P. Miškovský and A. Bertoluzza. Surface-enhanced resonance Raman spectroscopy of hypericin and emodin on silver colloids. SERRS and NIR FTSERS study. Biospectroscopy 1, 265 273 (1995).
  3. S. Sánchez-Cortes, P. Miškovský, D. Jancura and A. Bertoluzza. Specific interactions of antiretroviraly active drug hypericin with DNAas studied by surface-enhanced resonance Raman spectroscopy. J. Phys. Chem. 100, 1938–1944 (1996).
  4. S. Sánchez-Cortés, D. Jancura, P. Miškovský and A. Bertoluzza. Near infrared surface- enhanced Raman spectroscopic study of antiretroviraly drug hypericin and emodin in aqueous silver colloids. Spectrochimica Acta Part A 53, 769–779 (1997).
  5. G. Wheeler, P. Miškovský, D. Jancura and L. Chinsky. A study of metalloporphyrin polynucleotide interactions by microcalorimetry and circular dichroism. J. Biomol. Str. Dyn. 15, 967–985 (1998).
  6. P. Miškovský, D. Jancura, S. Sánchez-Cortés, E. Kočišová and L. Chinsky. Antiretroviraly active drug hypericin binds the IIA subdomain of human serum almumin. Resonance Raman and surface-enhanced Raman spectroscopy study. J.Am. Chem. Soc. 120, 6374–6379 (1998).
  7. D. Jancura, S. Sánchez-Cortes, P. Miškovský, L. Rivas, J. Staničová and J.V. García-Ramos. Surface-enhanced resonace Raman spectroscopy of hypocrellin A An effect of excitation wavelength and pH . Spectrochimica Acta Part A 54, 1519–1526 (1998).
  8. G. Fabriciová, P. Miškovský, D. Jancura and V. Lisý. Charcterization of low salt and high salt conformation of poly (dI-dC) by hydrogen-deuterium exchange kinetics A classical Raman spectroscopy study. J. Biomol. Str. Dyn. 16, 281–288 (1998).
  9. G. Fabriciová, P. Miškovský, D. Jancura, V. Lisý and L. Chinsky. Conformatiponal study of poly (dI-dC): Hydrogen-deuterium exchange and low frequency resonance Raman spectroscopy. J. Mol. Str. 480–481, 449–453 (1999).
  10. Kočišová, D. Jancura, S. Sánchez-Cortés, P. Miškovský, L. Chinsky and J.V. García- Ramos. Interaction of antiviral and antitumoral photoactive drug hypocrellin A with human serum albumin. J. Biomol. Str. Dyn. 17, 111–120 (1999).
  11. M. Fabian, D. Jancura and G. Palmer. Two sites of interaction of anions with cytocrome a in oxidized bovine cytochrome c oxidase. J. Biol. Chem. 279, 16170–16177 (2004).
  12. M. Fabian, L. Skultety, D. Jancura and G. Palmer. Implications of ligand binding studies for the catalytic mechamism of cytochrome c oxidase. BBA-Bioenergetics 1655,298–305 (2004).
  13. J.Hritz, J. Ulicny, A. Laaksonen, D. Jancura and P. Miskovsky. Molecular interaction model for the C1B domain of protein kinase C in the complex with its activator phorbol 12-myristate 13– acetate in water solution and lipid bilayer. J.Med.Chem. 47, 6547–6555 (2004).
  14. M. Antalik, D. Jancura, G. Palmer and M. Fabian. A role for the protein in internal electron transfer to the catalytic center of cytochrome c oxidase. Biochemistry 44, 14881–14889 (2005).
  15. S. Kascakova, M.Refregiers, D. Jancura, F. Sureau, J.C. Maurizot and P. Miskovsky. Fluorescence spectroscopic study of hypericin-photosensitized oxidation of low-density lipoproteins. Photochem.Photobiol. 81, 1395–1403 (2005).
  16. M. Fabian, D. Jancura, M. Bona, A. Musatov, M. Baran and G. Palmer. Influence of reduction of heme a and CuA on the oxidized catalytic center of cytochrome c oxidase: Insight from organic solvents. Biochemistry 45, 4277–4283 (2006).
  17. S. Kocanova, T. Hornakova, J. Hritz, D. Jancura, D. Chorvat jr., A. Mateasik, J. Ulicny, M. Refregiers, J.C. Maurizot and P. Miskovsky. Characterization of the interaction of hypericin witk protein kinase C in U-87 MG human glioma cells, Photochem. Photobiol. 82, 720–728 (2006).
  18. D. Jancura, M. Antalik, V. Berka, G. Palmer and M. Fabian. Filling the catalytic site of cytochrome c oxidase with electrons : Reduced CuB facilitates internal electron transfer to heme a3. J. Biol. Chem. 281, 20003–20010 (2006).
  19. D. Jancura, V. Berka, M. Antalik, J. Bagelova, R.B. Gennis, G. Palmer and M. Fabian. Spectral and kinetic equivalence of oxidized cytochrome c oxidase as isolated and „activated“ by reoxidation. J. Biol. Chem. 281, 30319–30325 (2006).
  20. M. Savko, S. Kascakova, P. Mojzes, D. Jancura, P. Miskovsky and J. Ulicny. Ground and excited state properties of naphthazarin: Absorption spectroscopy and theoretical modeling study, J.Mol.Struct. THEOCHEM 803, 79–87 (2007).
  21. S. Kascakova, Z. Nadova, A. Mateasik, J. Mikes, V. Huntosova, M. Refregiers, F. Sureau, J.C. Maurizot, P. Miskovsky and D. Jancura. High level of low-density lipoprotein receptors enhance hypericin uptake by U-87 MG cells in the presence of LDL. Photochem. Photobiol. 84, 120–127 (2008).
  22. P. Gbur, R. Dedic, D. Jancura, P. Miskovsky and J. Hala. Time resolved luminescence and singlet oxygen formation under illumination of hypericin in acetone. Journal of Luminescence, 128, 765–767 (2008),
  23. G.Lajos, D.Jancura, P.Miskovsky, J.V.Garcia-Ramos and S.Sanchez-Cortes. Surface-enhanced fluorescence and Raman scattering study of antitumoral drug hypericin: An effect of aggregation and self-spacing depending on pH. J.Phys.Chem. C 112, 12974–12980 (2008).
  24. P. Gbur, R. Dedic, D. Chorvat jr., P. Miskovsky, J. Hala and D. Jancura. Time resolved luminescence and singlet oxygen formation after illumination of hypericin-low-density lipoproteins complex. Photochem. Photobiol. 85, 816–8223 (2009).
  25. G. Lajos, D. Jancura, P. Miskovsky, J.V. Garcia-Ramos and S. Sanchez-Cortes. Interaction of the photosensitizer hypericin with low-density lipoproteins and phosphatidylcholine: A surface – enhanced Raman scattering and surface-enhanced fluorescence study. J. Phys. Chem. C 113, 7147–7154 (2009).
  26. V. Huntosova, L. Alvarez, L. Bryndzova, Z. Nadova, D. Jancura, L. Buriankova, S. Bonneau, D. Brault, P. Miskovsky and F. Sureau. Interaction dynamics of hypericin with low-density lipoproteins and U-87-MG cells. Int. J. Pharmaceutics 389, 32–40 (2010).
  27. L. Buriankova, Z. Nadova, D. Jancura, M. Refregiers, I. Yousef, J. Mikes and P. Miskovsky. Synchrotron based Fourier-transform infrared microspectroscopy as sensitive technique for the detection of early apoptosis in U-87 MG cells. Laser Phys. Lett. 7, 613–620 (2010).
  28. L. Buriankova, D. Buzova, D. Chorvat jr., F. Sureau, D. Brault, P. Miskovsky and D. Jancura. Kinetics of hypericin association with low-density lipoproteins. Photochem. Photobiol. 87, 56–63 (2011).
  29. G. Bano, J. Stanicova, D. Jancura, J. Marek, M. Bano, J. Ulicny, A. Strejckova and P. Miskovsky. On the diffusion of hypericin in dimethylsulfoxide/water mixtures-The effect of aggregation. J. Phys. Chem. B115, 2417–2423 (2011).
  30. V. Huntosova, D. Buzova, D. Petrovajova, P. Kasak, Z. Nadova, D. Jancura, F. Sureau and P. Miskovsky. Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells. Int. J. Pharmaceutics 436, 463–471 (2012).
  31. B. Buzova, V. Huntosova, P. Kasak, D. Petrovajova, J. Joniova, L. Dzurova, Z. Nadova, F. Sureau, P. Miskovsky and D. Jancura. Towards increased selectivity of drug delivery to cancer cells: development of a new LDL-based nanodelivery system for hydrophobic photosenzitizers. Proceedings of SPIE vol. 8460, Biosensing and Nanomedicine V, 84600U (2012).
  32. A. Strejckova, J. Stanicova, D. Jancura, P. Miskovsky and G. Bano. Spatial orientation and electric-field-driven transport of hypericin inside of bilayer lipid membranes. J. Phys. Chem. B 117, 1280–1286 (2013).
  33. E. Lopez-Tobar, M. Antalik, D. Jancura, M.V. Canamares, A. Garcia-Leis, D. Fedunova, G. Fabriciova and S. Sanchez-Cortes. Adsorption and detection of amyloid marker thioflavin T on Ag nanoparticles by surface-enhanced Raman scattering. J. Phys. Chem. C 117, 3996–4005 (2013).
  34. D. Petrovajova, D. Jancura, P. Miskovsky, D. Chorvat jr., A. Chorvatova, X. Ragas, M. Garcia-Diaz, S. Nonell and Z. Nadova. Monitoring of singlet oxygen luminescence and mitochondrial autofluorescence after illumination of hypericin/mitochondria complex: a time-resolved study. Laser Physics Letters 10, art. no. 075609 (2013).
  35. D. Buzova, P. Kasak, P. Miskovsky and D. Jancura. Solubilization of poorly soluble photosensitizer hypericin by polymeric micelles and polyethylene glycol. Gen. Phys. Bioph. 32, 201–208 (2013).
×

RNDr. Zuzana Jurašeková, PhD.

Senior researcher at the Department of Biophysics, Faculty of Science, Safarik University in Kosice.
M.S. degree in Biophysics and Chemical Physics in 2002 at Safarik University in Košice, Slovakia.
PhD degree in Physical Chemistry in 2009 at National University of Distance Education in Madrid, Spain.
Her present research interest is focused on the application of Raman scattering methods in the field of Cultural Heritage and Raman imaging.

Stages abroad

2003 (3 weeks): Charles University in Prague, Czech Republic
2005–2009 (4 years): IEM of CSIC in Madrid, Spain
2007; 2008; 2009/2010; 2010 (3 months; 1,5 month; 4 months; 4 months): ISOF of CNR in Bologna, Italy
2010 (1,5 month): The Metropolitan Museum of Art in New York, USA
2014 (3 months): IEM CSIC in Madrid, Spain
2015 (2 months): IEM CSIC in Madrid, Spain

Projects

VEGA 1/0929/16: Non invasive micro-Raman and SERS analysis of coloring materials in paper-based artifacts of cultural and historical interest: It is not a black as a black. (atRAM@Nt) – principal investigator

Publications

  • Garcia-Leis, A.; Jancura, D.; Antalik, M.; Garcia-Ramos, J. V.; Sanchez-Cortes, S.; Jurasekova, Z.: Catalytic effects of silver plasmonic nanoparticles on the redox reaction leading to ABTS˙+ formation studied using UV-visible and Raman spectroscopy. Phys. Chem. Chem. Phys. 18 (2016) 26562–26571.
  • Verebova, V.; Belej, D.; Joniova, J.; Jurasekova, Z.; Miskovsky, P.; Kozar, T.; Horvath, D.; Stanicova, J.; Huntosova, V.: Deeper insights into the drug defense of glioma cells against hydrophobic molecules. Int. J. Pharm. 503 (2016) 56–67.
  • Černák, J.; Helgesen,G.; Čižmár, E.; Kováč, J.; Jurašeková, Z.; Voltr, J.: Magnetic properties of graphene nanodisk and nanocone powders at low temperatures. Phys. Rev. B 92 (2015) 155429–1–7.
  • Jurasekova, Z.; Domingo, C.; Garcia-Ramos, J. V.; Sanchez-Cortes, S.: Effect of pH on the chemical modification of quercetin and structurally related flavonoids characterized by optical (UV-Visible and Raman) spectroscopy. Phys. Chem. Chem. Phys. 16 (2014) 12802–12811.
  • Guerrini L.; Jurasekova, Z.; del Puerto, E.; Hartsuiker, L.; Domingo, C.; Garcia-Ramos, J.V.; Otto, C.; Sanchez-Cortes, S.: The Effect of Metal-Liquid Interface Composition on the Adsorption of a Cyanine Dye onto Gold Nanoparticles. Langmuir 29 (2013) 1139–1147.
  • Jurasekova, Z.; Domingo, C.; Garcia-Ramos, J. V.; Sanchez-Cortes, S.: Adsorption and catalysis of flavonoid quercetin on different plasmonic metal nanoparticles monitored by SERS. J. Raman Spectrosc. 43 (2012) 1913–1919.
  • Jurasekova, Z.; Sanchez-Cortes, S.; Tamba, M.; Torreggiani, A.: Silver nanoparticles active as surface-enhanced Raman scattering substrates prepared by high energy irradiation. Vib. Spectrosc. 57 (2011) 42–48.
  • Jurasekova, Z.; Tinti, A.; Torreggiani, A.: Use of Raman spectroscopy for the identification of radical-mediated damages in human serum albumin. Anal. Bioanal. Chem. 400 (2011) 2921–2931.
  • Jurasekova, Z.; del Puerto, E.; Bruno, G.; Garcia-Ramos, J.V.; Sanchez-Cortes, S.; Domingo, C.: Extractionless non-hydrolysis Surface-Enhanced Raman Spectroscopic detection of historical mordant dyes on textile fibers. J. Raman Spectrosc. 41 (2010) 1455–1461.
  • Jurasekova, Z.; Marconi, M.; Sanchez-Cortes, S.; Torreggiani, A.: Spectroscopic and molecular modeling studies on the binding of the flavonoid luteolin and human serum albumin. Biopolymers 91 (2009) 917–927.
  • Torreggiani, A.; Jurasekova, Z.; D´Angelantonio, M.; Tamba, M.; Garcia-Ramos, J.V.; Sanchez-Cortes, S.: Fabrication of Ag nanoparticles by γ-irradiation: Application to surface-enhanced Raman Spectroscopy of fungicides. Colloids Surf. A Physicochem. Eng. Asp. 339 (2009) 60–67.
  • Jurasekova, Z.; Torreggiani, A.; Tamba, M.; Sanchez-Cortes, S.; Garcia Ramos, J.V.: Raman and Surface-Enhanced Raman Scattering (SERS) investigation of the quercetin interaction with metals: Evidence of structural changing processes in aqueous solution and on metal nanoparticles. J. Mol. Struct. 918 (2009) 129–137.
  • Jurasekova, Z.; Domingo, C.; Garcia-Ramos, J.V.; Sanchez-Cortes, S.: In situ detection of flavonoids weld dyed wool and silk textiles by Surface-enhanced Raman scattering. J. Raman Spectrosc. 39 (2008) 1309–1312.
  • Torreggiani, A.; Jurasekova, Z.; Sanchez-Cortes, S.; Tamba, M.: Spectroscopic and pulse radiolysis studies of the antioxidant properties of (+)-catechin: metal chelation and oxidizing radical scavenging. J. Raman Spectrosc. 39 (2008) 265–275.
  • Guerrini, L.; Jurasekova, Z.; Domingo, C.; Pérez-Méndez, M.; Leyton, P.; Campos-Vallette, M.; Garcia-Ramos, J.V.; Sanchez-Cortes, S.: Importance of Metal-Adsorbate Interactions for the Surface-enhanced Raman Scattering of Molecules Adsorbed on Plasmonic Nanoparticles. Plasmonics 2 (2007) 147–156.
  • Jurasekova, Z.; Garcia-Ramos, J.V.; Domingo, C.; Sanchez-Cortes, S.: Surface-enhanced Raman scattering of Flavonoids. J. Raman Spectrosc. 37 (2006) 1239–1241.
×

doc. RNDr. Martin Kundrát, PhD.

Associate Professor and Senior Research Associate
at Center for Interdisciplinary Biosciences
Faculty of Science, University of Pavol Jozef Šafárik in Košice.

Head of PaleoBioImaging Lab and Archosaur Evo-Devo Research Group

E-mail: martin.kundrat(at)upjs.sk
Lab Page: dinosaur.science.upjs.sk
Tel: +421(55) 234–2242
Fax: +421(55) 622–8109

Education:
2013 – Associate Professor in Zoology – Palacký University in Olomouc
2005 – PhD in Zoology – Chalrles University in Prague
2001 – Rerum Naturalium Doctoris in Zoology – Charles University in Prague
1992 – Mgr. in Biology-Chemistry – P. J. Šafárik University in Košice

Postdoctoral Research Training:
2007–2008 – Clemson University, Clemson, South Carolina, USA
2006–2007 – McGill University, Montreal, Quebec, Canada
2005–2006 – Macquarie University, Sydney, New South Wales, Australia

Languages:
English, Russian, Czech

Former Professional Affiliations:
2010–2015 – Researcher in Evolutionary Organismal Biology and Responsible for Archosaur Evo-Devo Research Program
Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden

2008–2010 – Senior Researcher in Vertebrate Paleobiology
Earth Science Institute, Slovak Academy of Sciences, Banská Bystrica

2003–2005 – Researcher in Evolutionary Morphology
Department of Zoology, Faculty of Natural Sciences, Charles University, Prague, Czech Republic

2003–2004 – Assitant in Human Histology and Embryology
Institute of Histolohy and Embryology, 1. Faculty of Medicine, Charles University, Prague, Czech Republic

1997–1998 – Head of Paleontological Research Station
Slovak National Museum, Bratislava

1996–1997 – Curator of Vertebrate Paleontology
Gemer-Malohont Museum, Rimavská Sobota

1992–1996 – Researcher in Zoology
Department of Anthropology and Zoology, Faculty of Natural Sciences, University of Pavol Jozef Šafárik, Košice

Lab Members – Postgraduate Students:
2013–2017 – MSc. Cazhi Shen, China
2016-present – MSc. Jason Hogan, USA

Current Projects (In Total: 28):
2017 – Evolution of flightlessness and gigantism in the island birds: model of the subrecent ornithofauna of New Zealand and Madagascar
Project ID: 1/0853/17
Position: Principal Investigator
Funding Source: VEGA, Slovakia

2017 – Evolutionary origin and innovations of skeleton-dental characters in Archosauria – Part 2
Project ID: 20165224
Position: Principal Investigator
Funding Source: Elettra Synchrotron, Italy

2016 – The evolution of flightless gigantic birds: quantitative reconstruction of 3D growth modules of moa (New Zealand) and the elephant bird (Madagascar) based on correlations with their closest recent relatives, tinamous and kiwis
Project ID: 32694
Position: Principal Investigator
Funding Source: Synchrotron Spring-8, Japan

2016 – Evolutionary origin and innovations of skeleton-dental characters in Archosauria
Project ID: 20155058
Position: Principal Investigator
Funding Source: Elettra Synchrotron, Italy

2015 – Quantitative analysis of bone microstructure in bird-like dinosaurs and early birds: implications to thermoregulation and paleogenomics
Project ID: 20150130
Position: Principal Investigator
Funding Source: Paul Scherrer Institut, Swiss Light Source, Switzerland

2015 – Exploring unique aspects of Australian Dinosaurs: (a) post-cranial structure of Leaellynasaura amicagraphica, and (b) locomotion of Triassic dinosauromorphs
Project ID: M8910
Position: Co-Investigator
Funding Source: The Australian Synchrotron, Australia

Expeditions (In Total: 15):
2014 – Leader – Henan Dinosaur Nesting Area Field Work, China
2013 – Leader – Iranian-Swedish Dinosaur Expedition, Iran
2013 – Leader – Siberian Early Tetrapod Expedition, Russia
2012 – Leader – Russian-Swedish Altay Jurassic Expedition, Russia
2011 – Leader – Xixia Dinosaur Egg Field Work, China
2011 – Leader – Siberian Early Tetrapod Expedition, Russia
2010 – Leader – Argentinian-Swedish Dinosaur Expedition, Argentina
2007 – Invited Researcher – Korean-Mongolian International Dinosaur Project, Mongolia
2005 – Invited Researcher – Dinosaur Provincial Park Field Work, Canada
1996 – Leader – Hajnáčka Expedition, Slovakia

New Extinct Taxa Designed:
2016 – the Lower Cretaceous dinosaur: Savannasaurus elliotorum, Queensland, Australia
2016 – the Upper Cretaceous dinosaur: Huanansaurus ganzhouensis, Ganzhou, China
2015 – the Upper Jurassic pterosaur: Orienthognathus chaoyangensis, Liaoning, China
2012 – the Upper Cretaceous pterosaur: Aerotitan sudamericanus, Patagonia, Argentina
2012 – the Upper Cretaceous dinosaur: Bonapartenykus ultimus, Patagonia, Argentina

Honors and Awards – Invited Positions:
2016 – Invited Fellow – Institute of Geology, Chinese academy of Geological Sciences Beijing, China
2016-present – Member of the Scientific Board for Postgraduate Studies in Paleontology – Department of Geology and Paleontology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
2015-present – Editorial Board: Historical Biology – An International Journal of Paleobiology; Publisher: Taylor & Francis
2013 – Invited Researcher – European Synchrotron Radiation Facility, Paris, France
2012 – Invitation for writing peer-reviewed chapter in presitgious scientific book (see below)
2012 – Invited Researcher – European Synchrotron Radiation Facility, Paris, France
Versita, co-published with Springer-Verlag
2009 – ESRF Paleo-Initiative Group – European Synchrotron Radiation Facility, Grenoble, France
2009 – Invited Professor – Muséum national d’Histoire naturelle, Paris, France
2009 – Invited Fellow – Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing, China
2008 – Invited Professor – Muséum national d’Histoire naturelle, Paris, France
2007–2009 – Editor of The Central European Journal of Biology; Publisher: Versita, co-published with Springer-Verlag
2004 – Welcomme Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology Award – California University in San Fracisco, USA
2003–2004 – NATO Science Fellowship Programm Winner – Wesleyan University, Connecticut, USA
1992 – P.J. Šafárik University´s Rector award for outstanding study results, Košice, Slovakia

Selected Invited Lectures (In Total: 38):
2016 Jul 12 – Jiangxi Professional College of Applied Technology, Guanzhou, China
2015 Mar 24 – Canterbury Museum, Christchurch, New Zealand
2014 Nov 26 – Chinese Academy of Geological Sciences, Beijing, China
2014 Nov 21 – Plenary Lecture at Zoológia 2014 Congress, Prešov, Slovakia
2013 Dec 5 – Korea-Mongolia International Dinosaur Symposium, South Korea
2013 Sep 23 – Tehran University, Tehran, Iran
2012 Nov 22 – Plenary Lectue at Zoológia 2012 Congress, Zvolen Slovakia
2011 May 27 – Abteilung Molekulare Embryologie, Universität Freiburg, Germany
2009 Dec 14 – Evolutionary Biology Center, Uppsala University, Uppsala, Sweden
2009 Oct 13 –European Synchrotron Radiation Facility, Grenoble, France
2009 Jun 4 – Museum National d’Histoire Naturelle, Paris, France
2009 May 26 – Department of Biological Sciences, University of Calgary, Canada
2009 Apr 11 – Goseong Dinosaur World Expo 2009 – Goseong International Dinosaur Symposium, South Korea
2008 Sep 24 – Royal Tyrrell Museum of Palaeontology, AB, Canada
2007 Sep 28 – Institute of Vertebrate Paleontology and Paleoanthropology, Beijing, China
2005 Dec 8 – Australian Museum, Sydney, Australia
2005 Nov 3 – Department of Biological Science, Macquarie University, Sydney, Australia
2005 Sep 2 – Museum National d’Histoire Naturelle, Paris, France
2005 Jun 13 – Department of Biological Sciences, University of South Carolina, Columbia, USA
2004 Apr 14 – Department of Orthopaedic Surgery, University of California at San Francisco, USA
2004 Mar 23 – Department of Biological Sciences, Wesleyan University, Middletown, USA
2003 Jul 4 – Laboratory for Evolutionary Morphology, Center for Developmental Biology, RIKEN, Kobe, Japan
2001 Sep 21 – Department of Biological Sciences, University of Calgary, Canada

Invited Major Interviews (In Total: 24):
2016 – by T. Watson, USA Today
2015 – by M. Balter, Science
2015 – by V. Richter, Cosmos Magazine, Australia
2015 – by E. Spink, The Dominion Post, New Zealand
2016 – by Ch. Yang, Xinhua News Agency of China
2014 – by E. Callaway, Nature
2009 – by D. Chinoy, China Daily
2008 – by J. Matejíčková, Quark, Slovakia
2004 – by E. Pennissi, Science
2004 – by S. Freeman, The Republican, USA

Selected Peer-Reviewed Publications (In Total: 33; Citations: 306; HirIn: 10):
2017 – Pu, H., Currie, P. J., Lü, J., Zelenitsky, D. K., Carpenter, K., Li, X., Koppelhus, E. B., Jia, S., Xiao, L., Chuang, H., Li, T., Kundrát, M., Shen, C. Perinate and eggs of a giant caenagnathid dinosaur from the Late Cretaceous of central China. Nature Communications (accepted).
IF: 11.329

2016 – Lü, J., Kundrát, M., Shen, C. A reappraisal of the morphology, histology, and systematic position of the pterosaur Gladocephaloideus from the Early Cretaceous of Liaoning Province, China. Plos ONE 11(6): e0154888.
IF: 3.540

2016 – Poropat, S. F., Mannion, P. D., Upchurch, P., Hocknull, S. A., Kear, B. P., Kundrát, M., Tischler, T. R., Sloan, T., Sinapius, G. H. K., Elliot, J. A., Elliott, D. A. New Australian sauropod dinosaurs elucidate Cretaceous biogeography. Scientific Reports 6: 34467.
IF: 5.228

2016 – Kundrát, M. Great Transformations in Vertebrate Evolution edited by Kenneth P. Dial, Neil Shubin and Elizabeth L. Brainerd. Systematic Biology 65(2): 349–352.
IF: 14.387

2015 – Lü, J., Pu, H., Kobayashi, Y., Xu, L., Chang, H., Shang, Y., Liu, D., Lee, Y-N., Kundrát, M., Shen, C. A new oviraptorid dinosaur (Dinosauria: Oviraptorosauria) from the Late Cretaceous of southern China and its paleobiogeographical implications. Scientific Reports 5: 11490.
IF: 5.078

2015 – Fernandez, V., Buffetaut, E., Suteethorn, V., Rage, J-C., Tafforeau, P., Kundrát, M. (corresp. author). Evidence of egg diversity in squamate evolution from Cretaceous anguimorph embryos. PloS ONE 10(7): e0128610.
IF: 3.534

2012 – Kundrát, M. Phenotypic and geographic diversity of the European lesser panda Parailurus. In Red Panda – Biology and Conservation of the First Panda (ed. A. R. Glatston), 61–88, Elsevier, Academic Press: Boston, Heidelberg, London, New York, Oxford, Paris, San Diego, San Francisco, Singapore, Sydney, Tokyo.

2009 – Kundrát, M. Heterochronic shift between early organogenesis and migration of cephalic neural crest cells in the two divergent evolutionary archosaur phenotypes: crocodile and ostrich. Evolution & Development 11(5): 535–546.
IF: 3.627

2009 – Kundrát, M. Primary chondrification foci in the wing basipodium of Struthio camelus with comments on interpretation of autopodial elements in Crocodilia and Aves. Journal of Experimental Zoology (Molecular Developmental Evolution) 312B(1): 30–41.
IF: 3.364

2008 – Kundrát, M. (corresp. author), Joss, J., Smith, M. M.. Fate mapping in embryos of Neoceratodus forsteri reveals cranial neural crest participation in tooth development as conserved from lungfish to tetrapods. Evolution & Development 10(5): 531–536.
IF: 3.733

2007 – Kundrát, M. 2007. Avian-like attributes of a virtual brain model of the oviraptorid theropod Conchoraptor gracilis. Naturwissenschaften 94(6): 499–504.
IF: 2.021

2003 – Galis, F., Kundrát, M., Sinervo, B. 2003. An old controversy solved: Bird embryos have five fingers. Trends in Ecology & Evolution 18(1): 7–9.
IF: 11.929

Selected Science Popularization Articles (In Total: 43)
2016 – Kundrát, M. Titáni pavekej Austrálie. Quark 12: 28–29. [in Slovak]
2016 – Kundrát, M. Pravek vo vysokom rozlíšení (venované Prof. Zdeňkovi V. Špinarovi). Quark 5: 27–29. [in Slovak]
2016 – Kundrát, M. Nájdený ale prehliadnutý: ako som objavil zub Homo erectus pekinensis. Quark 1: 28–29. [in Slovak]
2015 – Kundrát, M. Na love v čínskom praveku. Quark 11: 22–24. [in Slovak]
2015 – Kundrát, M. Odkedy žijú na Slovensku vtáky? Quark 2: 24–25. [in Slovak]
2014 – Kundrát, M. Argentinský úsvit dinosaurov – časť prvá: Talampaya. Quark 12: 23–26. [in Slovak]
2013 – Kundrát, M. Titani pravekého neba. Quark 2: 24–25. [in Slovak]
2012 – Kundrát, M. Pôvabný operený tyran. Quark 6: 20–21. [in Slovak]
2011 – Kundrát, M. V hniezdisku čínskeho draka. Quark 12: 32–33. [in Slovak]
2010 – Kundrát, M. Raptor – viac ako plaz a menej ako vták. Quark 5: 26–27. [in Slovak]
2009 – Kundrát, M. Evolučné posolstvo Darwina. Quark 6 (príloha): 1–8. [in Slovak]
2008 – Kundrát, M. Žili dinosauri aj u nás? Quark 4: 22. [in Slovak]

×

doc., RNDr. Erik Sedlák, PhD.

Associate professor at Department of Chemistry, Faculty of Natural Sciences, Safarik University in Kosice (UPJS), PhD. degree in Biochemistry (1998), UPJS, associate professor in Chemistry at UPJS from 2006. Specialist in protein chemistry and enzymology. Recent research interests: structure and stability of biomacromolecules and their complexes with low molecular ligands; thermodynamics and kinetics stability of proteins such as IgGs, DARPins, GOX, LOV domain, and membrane proteins; Hofmeister effect on properties of enzymes, particularly proteases.

Education:
1976 – 84 Elementary school, ZS Gottwaldova/ZS Studentska Snina
1984 – 88 High school, Gymnázium Snina
1988 – 93 Safarik University Kosice (Slovakia) – MSc. in Biophysics
1993 – 97 Safarik University Kosice – Ph.D. study in Biochemistry
1998 – UPJS – Defence of Ph.D. thesis
2006 – Habilitation to Associate Professor of chemistry at Department of Biochemistry PF UPJS

Languages
English, Slovak

Professional positions:
1996–1998 Research Associate in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
1999–2001 Research Scientist & Faculty Member in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2002–2007 Research Scientist & Faculty Member in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2003–2007 Head of Department of Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2007–2013 Several continual foreign stays
2010–2011 Associate Editor for Biochemistry in Chemical papers (Bratislava)
2011–2013 Editor in Chemical papers (Bratislava)
2013-presence Senior Research Associate at Centre for Interdisciplinary Biosciences, P.J. Šafárik University, Košice, Slovak Republic, Associate Professor at Department of Biochemistry, , P.J. Šafárik University, Košice, Slovak Republic
2018-presence Head of Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Šafárik University, Košice, Slovak Republic

Postdoctoral research training:
1994 (1 month), 1995 (3 months), 1997 (1 month), 2000 (2 months), 2002 (1 month) 2004 (1 month), 2005 (1 month) Laboratorium für Biochemie, Universität Bayreuth, Germany, with Prof. Dr. Mathias Sprinzl, Dr.h.c.
1998–1999 (21 months) Postdoctoral Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof. Neal C. Robinson. Project: „Functional Association of Cardiolipin with Cytochrome c Oxidase and bc1.“
2001–2002 (12 months) Postdoctoral Senior Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof.
2003 (3 months) Neal C. Robinson. Project: „Function of Cardiolipin in Cytochrome c Oxidase and bc1.“
2007–2008 (18 months) Postdoctoral Senior Research Associate in Biochemistry, The Rice University, Houston, USA, with Assoc. Prof. Dr. Pernilla Wittung-Stafshede. Project: „Folding mechanisms of copper transport proteins.“
2008–2009 (12 months) Postdoctoral Senior Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof. Neal C. Robinson. Project: “The functional and structural role of phospholipids bound to mitochondrial complexes.”
2009–2013 (46 months) Postdoctoral Associate in Biochemistry, The University of Zürich, Zürich, Switzerland with Prof. Dr. Andeas Plückthun. Projekt: “ Conversion of integral membrane receptors into soluble forms ” (Marie-Curie Intra-European Fellowship)
2014 (4 months) Visiting Researcher in laboratory of Prof. Dr. Andeas Plückthun, Department of Biochemistry, The University of Zürich, Zürich, Switzerland
2016 (2 months) Visiting Researcher in laboratory of Prof. Dr. Andeas Plückthun, Department of Biochemistry, The University of Zürich, Zürich, Switzerland

Honor and awards
• The best scientific work or collection of works of young scientist (under 35 years) in biophysics (granted by Slovak Biophysical Society; 2004)
• Award of Minister of Education of Slovak Republic for Science and Technique in category of Established results of young workers in science and development to 35 years; 2004.
• Reception by President of Slovak Republic; January 19th, 2005.
• Marie-Curie Intra-European Fellowship (~200,000 Eur) (2009–2011) in laboratory of Prof. Dr. Andreas Plückthun, University of Zürich. Project: “ Conversion of integral membrane receptors into soluble forms

Technical expertise:
Technical Expertise:

(i) Ribosome and Yeast displays – directed evolution methods for peptides/proteins.
(ii) Spectral techniques – absorbance spectroscopy, fluorescence and circular dichroism.
(iii) Differential scanning calorimetry – determination of thermodynamic parameters from microcalorimetric measurements of reversible and irreversible transitions; isothermal titration calorimetry.
(iv) Chromatography and analytical techniques (HPLC, FPLC), ELISA, Western blotting, SDS PAGE, with experience in analysis of experiments on analytical ultracentrifugation (from Center for Analytical Ultracentrifugation of Macromolecular Assemblies (CAUMA) in San Antonio).
(v) Protein purification (from heart tissue – cytochrome c oxidase, from bacteria – translation, elongation factors, heterologously expressed proteins).
(vi) Basic molecular biology, biochemical and biophysical methods.

Scientific projects (E.S. principal or co-principal investigator):

I. Science grant agency of the Ministry of education of the SR and of the Slovak Academy of Sciences (VEGA) (6)
2017–2019 VEGA 2/0009/17 (co-principal investigator)
Project: Oxidative stress and phospholipid-protein interactions: functional and structural consequences
2014–2016 VEGA 2/0062/14 (11 388 €, co-principal investigator)
Project: Functionality and Structural Integrity of Proteins in Bicelles – Implications for Mitochondrial and Amyloidogenic Proteins
2012–2014 VEGA 1/0521/12 (6 225 €, co-principal investigator)
Project: Modulation of protein dynamics and catalytical properties of enzymes by
Hofmeister ions.
2007–2009 VEGA 1/4319/07 (18 787 €, co-principal investigator)
Project: Study of conformational, functional, and sequentional diversities of
flavoproteins and chosen homodimeric oxidases.
2006–2008 VEGA 1/3252/06 (17 991 €, principal investigator)
Project: Stability and dynamics of chosen multidomain translational factors from
thermophile bacteria Thermus thermophilus.
2004–2006 VEGA 1/1272/04 (18 190 €, co-principal investigator)
Project: Effect of polyanions on structure, stabilty, and solubility of proteins.
2003–2005 VEGA 1/0432/03 (22 737 €, principal investigator)
Project: Modulation of structural and functional properties of flavoproteinoxidases
from thermophile Thermus thermophilus and mesophile Aspergillus niger by
modification of solvent properties.
II. Slovak Research and Development Agency (APVV) (1)
2016–2020 APVV 15–0069 (170 000 €, principal investigator)
Project: Conversion of integral membrane protein into water soluble form: the case of GPCR
III. Other grants:
2009–2011 Marie-Curie Intra-European Fellowship (~200 000 €, principal investigator)
2005 Deutsche Forschungsgemeinschaft in collaboration with Prof.Dr. Mathias Sprinzl, Dr.h.c., Universität Bayreuth, Germany (12 613 €, co-principal investigator)
2005 Development project awarded by Ministry of education of Slovak Republic (17 426 €, principal investigator)

Invited congress lectures:
7 lectures (international congresses) including 3 plenary lectures
Graduated PhD students:
• 2014 Kamil Tóth (thesis defense: UPJS)
• 2011 Nataša Tomášková (thesis defense: UPJS)
• 2006 Gabriel Žoldák (as co-supervisor, thesis defense: UPJS)
Diploma students:
15 finished, 2 in the process.
Publications:
63 publications in international peer-reviewed journals (see WOS and Scopus databases), cited more than 780 times, Hirsch index 19
Kosice, Januar, 2018

×

Dr. Shubhashis Datta, PhD.

Email: shubhashis.datta@upjs.sk

Postdoctoral researcher at the Center for Interdisciplinary Biosciences, P.J. Safarik University, Kosice, Slovakia under Prof Pavol Miskovsky since May 2016. Specialist in biophysical techniques and optical spectroscopy. Present research focus is on the development of lipoprotein based Nano-construct for cancer targeting.

EDUCATION:
Ph.D. (Graduate) Research: From January, 2009 to January, 2015 at the Department of Chemistry, Indian Institute of Technology, Kharagpur under the guidance of Prof Mintu Halder on studies on site-specific interaction of fluorophores and drug molecules with serum albumins and their possible applications.
M.Sc.: From 2006 to 2008 at the Department of Chemistry and Chemical Technology, Vidyasagar University, West Bengal, India.

Postdoc Research Experience:
From January 2015 to January 2016 as a postdoctoral research fellow at the department of Chemistry, Hebrew University of Jerusalem, Israel on the development of sensor for the early detection of cancer biomarker.

PUBLICATIONS:
(1) Shubhashis Datta, S. Panja, P, Mitra and M, Halder. ‘’Distilbene Derivative as New Environment Sensitive Bifunctional Ligand for Possible Induction of Serum Protein Aggregation: A Spectroscopic Investigation and Potential Consequences. Langmuir (Highlighted in Nature-India), Vol-31, p. 10781–90 (2015).

(2) Shubhashis Datta, S. Panja, M. Halder. “Detailed scenario of the acid-base behavior of prototropic molecules in the subdomain-IIA pocket of serum albumin: Results and prospects in drug delivery” J. Phys. Chem. B 2014, 118,12153–12167

(3) Shubhashis Datta, M. Halder. “Detailed scrutiny of the anion receptor pocket in subdomain IIA of serum proteins toward individual response to specific ligands: HSA-Pocket resembles flexible biological slide-wrench unlike BSA” J. Phys. Chem. B 2014, 118, 6071–6085.

(4) Shubhashis Datta, M. Halder. “Effect of encapsulation in the anion receptor pocket of subdomain IIA of human serum albumin on the modulation of pKa of warfarin and structurally similar acidic guests: A possible implication on biological Activity” J. Photochem. Photobiol. B 2014, 130, 76–85. (Highlighted in Nature-India)

(5) Shubhashis Datta, N. Mahapatra, M. Halder. “pH-insensitive electrostatic interaction of carmoisine with two serum proteins: A possible caution on its uses in food and pharmaceutical industry” J. Photochem. Photobiol. B 2013, 124, 50–62.

(6) P. Bolel, Shubhashis Datta, N. Mahapatra, M. Halder. “Exploration of pH-dependent behavior of the anion receptor pocket of subdomain IIA of HSA: Determination of effective pocket charge using the Debye−Hückel limiting law” J. Phys. Chem. B 2014, 118, 26–36.

(7) P. Bolel, Shubhashis Datta, N. Mahapatra, M. Halder. “Spectroscopic investigation of the effect of salt on binding of tartrazine with two homologous serum albumins: Quantification by use of the Debye−Hückel limiting law and observation of enthalpy−entropy compensation” J. Phys. Chem. B 2012, 116, 10195–10204.

(8) P. Bolel, N. Mahapatra, Shubhashis Datta, M. Halder. “Modulation of accessibility of subdomain IB in the pH-dependent interaction of bovine serum albumin with Cochineal Red A: A combined view from spectroscopy and docking simulations” J. Agric. Food Chem. 2013, 61, 4606–4613.

(9) N. Mahapatra, Shubhashis Datta, M. Halder. “A new spectroscopic protocol for selective detection of water soluble sulfides and cyanides: Use of Ag-nanoparticles synthesized by Ag (I)–reduction via photo-degradation of azo-food-colorants” J. Photochem. Photobiol. A 2014, 275, 72–80.

(10) Shubhashis Datta, P. Bolel, N. Mahapatra, S. Panja, H. Vardhan, S. Kayal, D. Khatua, I. Das and Mintu Halder. Reorganization Energy and Stokes Shift Calculations from Spectral Data as New Efficient Approaches in Distinguishing the End Point of Micellization/Aggregation, Analytical Methods, Vol-8, p. 2805–2811 (2016).

(11) M. Rajabi, Z. Hossaini, M. A. Khalilzadeh, Shubhashis Datta, Mintu Halder, S. A. Mousa. Synthesis of a new class of fluro [3,2-c] coumarins and its anticancer activity J. Photochem. Photobiol B: Biology, Vol-148, p. 66–72 (2015).

ATTENDED CONFERENCE

  1. Published abstract and presented poster entitled “Exploration of Specific Interaction of Food Dyes with Two Homologous Serum Proteins and Its Possible Biological Implications” in the International Symposium named “Theme Meeting on Ultrafast Science, (UFS-2013)” organized by Department of Physics and Meteorology, IIT Kharagpur, and Indian Society for Radiation and Photochemical Sciences, Mumbai during October 25–26, 2013 at IIT Kharagpur, India.
  2. Attended the International Symposium named “Light in Chemistry, Materials and Biology, (LCMB-2014)” organized by Department of Chemistry, IIT Kharagpur, Kharagpur during February 24–25, 2014 at Kharagpur, India.
  3. Attended the International Symposium named “Diamond Jubilee Symposium on Recent Trends in Chemistry, (DJSRTC-2011)” organized by Department of Chemistry, IIT Kharagpur, Kharagpur during October 21–23, 2011 at IIT Kharagpur, India.
×

MSc. Alba Espina García

e-mail: albaespinagarcia@gmail.com

Graduated (Bachelor of Arts) in Conservation and Restoration of Culture Heritage at Escuela Superior de Arte del Principado de Asturias (ESAPA), Spain (2014).

MSc degree in Science and Technology of Materials at University of Oviedo, Spain (2016). Short-term study/research stay (3 months, 2016) at Instituto de Estructura de la Materia, CSIC (Madrid), Spain. It was concerned on developing the Master thesis project: Vibrational characterization of synthetic pigments of historical interest to the cultural heritage by spectroscopic techniques based on non-destructive optical plasmonic effects.

Since November 2016, the intern at Center for Interdisciplinary Biosciences, P. J. Safarik University in Kosice, Slovakia. She is working on the project focused on the black inks and their non-invasive Raman and SERS analysis under supervision of dr. Jurasekova.

×

Msc. Quentin Monfroy

Ph.D. student (1st year)
at Department of Animal Physiology, Faculty of Sciences
University Pavol Jozefa Šafárika of Košice

Member (Postgraduate Student) of Kundrát’s PaleoBioImaging Lab and Evolutionary Biodiversity Research Group
At Center for Interdisciplinary Biosciences, Technology and Innovation Park
University Pavol Jozefa Šafárika of Košice
dinosaur.science.upjs.sk

Educational Background
Since 09/2017: Doctoral studies – Pavla Jozefa Šafárika University in Košice – Košice (Slovakia)
• Research subject: Analysis of bone microstructure – in search of correlations with thermophysiology of the recent and extinct Amniota
09/2014 – 07/2016: Master of Science: Biology – University of Rennes 1 – Rennes (France)
• Research subjects: Ootaxonomy, Mesozoic Crocodilian and Marine Reptile Bone Identification, Theropod Functional Morphology.

Work Experience
08/2016 – 06/2017 : Collection Manager at the Musée d’Art et d’Histoire de Sainte-Ménehould Sainte Ménehould (France)
• Inventory of a Paleontology Collection from the 19th Century – Identification, Classification and Database Creation of fossils from French and International Sites.
• Simultaneously worked as project manager for a WWI photo exhibit, assisting the American photographer, Dr. Jeffrey Gusky, both in the field and the museum.

Publications
Monfroy, Quentin T., 2017: Correlation between the size, shape and position of the teeth on the jaws and the bite force in Theropoda. Historical Biology, Vol. 29, Iss. 8, 2017, p. 1089–1105. (available online: http://www.tandfonline.com/…2017.1286652)

Languages
English and French (Bilingual and Bicultural), German

Professional Experience
01/2016 – 06/2016: Royal Tyrrell Museum of Palaeontology, Drumheller – Alberta (Canada)
• Study of dinosaur and crocodilian skulls for Master’s thesis.
06/2016 – 07/2014: Grube Messel – Frankfurt am Main (Germany)
• Excavation of fossilized plants, invertebrates and vertebrates from the Paleocene-Eocene area in bituminous layers of shale.
06/2014 – 07/2014: University of Calgary – Alberta (Canada)
• 3D modelling with CT scans of Amphibians.
• Excavation at a Pachyrhinosaurus site last dug during the 1950s.
06/2013 – 07/2013: Grotte du Noisetier – Fréchet-Aure (France)
• Excavation of animal remains and artifacts from the last Ice Age and Homo neanderthalensis.
06/2012 – 07/2012: Egg Mountain – Montana (U.S.A.)
• Excavation of reptile, mammal and dinosaur fossils from the Cretaceous period.
08/2011 – 09/2011: Bassin d’Autun – Muse (France)
• Excavation of marine animal and plant fossils from the Permian period.
07/2011: Site de Dinoplagne – Plagne (France)
• Excavation, cleaning and protection of Sauropod and small Theropod tracks from the Late Jurassic period.

×

RNDr. Gabriel Žoldák, PhD.

Specialist in protein stability and folding, single molecule protein biophysics, optical tweezers, protein thermodynamics and kinetics. Recent research interests: force spectroscopy of heat shock proteins 70, chaperones, cis/trans proline isomerisation, conformational stability of therapeutic proteins.

Education:
09/1998 – 07/2001 PJ Safarik University Kosice (Slovakia) – Bc. in Chemistry
09/2001 – 06/2003 PJ Safarik University Kosice (Slovakia) – Msc. in Biochemistry
09/2003 – 05/2006 PJ Safarik University Kosice – Ph.D. study in Biochemistry
05/2006 Defence of Ph.D. thesis

Languages
German, English, Slovak

Professional positions:
2006 Researcher in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2006–2010 Postdoctoral Scientists, University Bayreuth, Dep. Biochemistry, Germany
2010– 2017 Principal Investigator, Technical University Munich, Germany
2010–2017 Team Leader, University Bayreuth, Dep. Biochemistry, Germany
2012–2016 Associate Editor for Biochemistry in Chemical papers (Bratislava)
2017-presence Researcher at Centre for Interdisciplinary Biosciences, P.J. Šafárik University, Košice, Slovak Republic

Grants and Projects
01/2016–12/2017 DAAD 57212263, bilateral exchange program GER/CZ, Protein-protein interaction within the 14–3–3 family. EUR 8.000
08/2010–08/2012 ZO-291/1–1 DFG, German research council, Communication between the domains of the DnaK chaperone characterized by single molecule force spectroscopy. EUR 160.000
01/2007–12/2007 VEGA 1/4319/07, Study of conformational, functional and sequence diversity of homodimeric flavoproteins, EUR ca. 8.000

Publications

Researcher ID: Q-7412–2017, ORCID ID: orcid.org/0000–0002–5271–8837,
Scopus Author ID: 6506775027

Publications: 33, Citations: 607 (Scopus/WOS), h-index 14, * corresponding author, 1st-first author, iequal contribution with the first author

  1. Garajová K, Zimmermann M, Petrenčáková M, Dzurová L, Nemergut M, Škultéty Ľ, Žoldák G, Sedlák E. The molten-globule residual structure is critical for reflavination of glucose oxidase. Biophys Chem. 2017 Nov;230:74–83.
  2. Nemergut M, Žoldák G, Schaefer JV, Kast F, Miškovský P, Plückthun A, Sedlák E. Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering. Protein Sci. 2017 Nov;26(11):2229–2239
  3. Žoldák G, Jancura D, Sedlák E. The fluorescence intensities ratio is not a reliable parameter for evaluation of protein unfolding transitions. Protein Sci. 2017 Jun;26(6):1236–1239.
  4. Mandal SS, Merz DR, Buchsteiner M, Dima RI, Rief M, Žoldák* G. Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change. Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):6040–6045.
  5. Pelz B, Žoldák G, Zeller F, Zacharias M, Rief M. Subnanometre enzyme mechanics probed by single-molecule force spectroscopy. Nat Commun. 2016 Feb 24;7:10848.
  6. Bauer D, Merz DR, Pelz B, Theisen KE, Yacyshyn G, Mokranjac D, Dima RI, RiefM, Žoldák* G. Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK. Proc Natl Acad Sci USA. 2015 Aug 18;112(33):10389–94.
  7. Hocking HG, Häse F, Madl T, Zacharias M, Rief M, Žoldák* G. A compact native 24-residue supersecondary structure derived from the villin headpiece subdomain. Biophys J. 2015 Feb 3;108(3):678–86.
  8. Rognoni L, Möst T, Žoldák G, Rief M. Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5568–73.
  9. Žoldák G, Rief M. Single molecule analysis: Force spectroscopy – Dynamics and manipulation of single molecules(Article)[Kraftspektroskopie – Dynamik und Manipulation einzelner Moleküle: Einzelmolekülanalyse] BioSpektrumVolume 20, Issue 1, February 2014, 33–35
  10. Li J, Žoldák G, Kriehuber T, Soroka J, Schmid FX, Richter K, Buchner J. Unique proline-rich domain regulates the chaperone function of AIPL1. Biochemistry. 2013 Mar 26;52(12):2089–96.
  11. Žoldák G, Rief M. Force as a single molecule probe of multidimensional protein energy landscapes. Curr Opin Struct Biol. 2013 Feb;23(1):48–57.
  12. Žoldák* G, Stigler J, Pelz B, Li H, Rief M. Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy. PNAS U S A. 2013 Nov 5;110(45):18156–61.
  13. Žoldák G, Geitner AJ, Schmid FX. The prolyl isomerase SlyD is a highly efficient enzyme but decelerates the conformational folding of a client protein. JACS. 2013 Mar 20;135(11):4372–9.
  14. Carstensen L, Žoldák G, Schmid FX, Sterner R. Folding mechanism of an extremely thermostable (βα)(8)-barrel enzyme: a high kinetic barrier protects the protein from denaturation. Biochemistry. 2012 Apr 24;51(16):3420–32.
  15. Žoldák G, Schmid FX. Cooperation of the prolyl isomerase and chaperone activities of the protein folding catalyst SlyD. J Mol Biol. 2011 Feb 11;406(1):176–94.
  16. Tóth K, Sedlák E, Musatov A, Žoldák* G. Activity of NADH oxidase from Thermus thermophilus in water/alcohol binary mixtures is limited by the stability of quaternary structure. Journal of Molecular Catalysis B: Enzymatic Volume 2010, Jun; 64: 60–67
  17. Žoldák G, Aumüller T, Lücke C, Hritz J, Oostenbrink C, Fischer G, Schmid FX. A library of fluorescent peptides for exploring the substrate specificities of prolyl isomerases. Biochemistry. 2009 Nov 3;48(43):10423–36.
  18. Žoldák G, Carstensen L, Scholz C, Schmid FX. Consequences of domain insertion on the stability and folding mechanism of a protein. J Mol Biol. 2009 Mar 6;386(4):1138–52.
  19. Jakob RP, Žoldák G, Aumüller T, Schmid FX. Chaperone domains convert prolyl isomerases into generic catalysts of protein folding. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20282–7.
  20. Weininger U, Haupt C, Schweimer K, Graubner W, Kovermann M, Brüser T, Scholz C, Schaarschmidt P, Žoldák G, Schmid FX, Balbach J. NMR solution structure ofSlyD from Escherichia coli: spatial separation of prolyl isomerase and chaperone function. J Mol Biol. 2009 Mar 27;387(2):295–305.
  21. Sedlák E, Žoldák G, Wittung-Stafshede P. Role of copper in thermal stability of human ceruloplasmin. Biophys J. 2008 Feb 15;94(4):1384–91.
  22. Žoldák G, Sedlák E, Wolfrum A, Musatov A, Fedunová D, Szkaradkiewicz K, Sprinzl M. Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains. Biochemistry. 2008 Apr 29;47(17):4992–5005.
  23. Tóth K, Sedlák E, Sprinzl M, Žoldák* G. Flexibility and enzyme activity of NADH oxidase from Thermus thermophilus in the presence of monovalent cations of Hofmeister series. Biochim Biophys Acta. 2008 May;1784(5):789–95.
  24. Žoldák G, Redecke L, Svergun DI, Konarev PV, Voertler CS, Dobbek H, Sedlák E, Sprinzl M. Release factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies. Nucleic Acids Res. 2007;35(4):1343–53.
  25. Tomásková N, Varhac R, Žoldák G, Oleksáková L, Sedláková D, Sedlák E. Conformational stability and dynamics of cytochrome c affect its alkaline isomerization. J Biol Inorg Chem. 2007 Feb;12(2):257–66.
  26. Žoldák G, Sedlák E, Valusová E, Wolfrum A, Marek J, Antalík M, Sprinzl M. Irreversible thermal denaturation of elongation factor Ts from Thermus thermophilus effect of the residual structure and intermonomer disulfide bond. Biochim Biophys Acta. 2006 Jul;1764(7):1277–85.
  27. Hritz J, Žoldák G, Sedlák E. Cofactor assisted gating mechanism in the active site of NADH oxidase from Thermus thermophilus. Proteins. 2006 Aug1;64(2):465–76.
  28. Stupák M, Žoldák G, Musatov A, Sprinzl M, Sedlák E. Unusual effect of salts on the homodimeric structure of NADH oxidase from Thermus thermophilus in acidic pH. Biochim Biophys Acta. 2006 Jan;1764(1):129–37.
  29. Žoldák G, Musatov A, Stupák M, Sprinzl, M., Sedlák E. pH-induced changes in activity and conformation of NADH oxidase from Thermus thermophilus. General Physiology and Biophysics 2005 (3) , 279–298
  30. Žoldák G, Zubrik A, Musatov A, Stupák M, Sedlák E. Irreversible thermal denaturation of glucose oxidase from Aspergillus niger is the transition to the denatured state with residual structure. J Biol Chem. 2004 Nov12;279(46):47601–9.
  31. Žoldák G, Sprinzl M, Sedlák E. Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions. Eur J Biochem. 2004 Jan;271(1):48–57.
  32. Žoldák G, Sut'ák R, Antalík M, Sprinzl M, Sedlák E. Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus. Eur J Biochem. 2003 Dec;270(24):4887–97.
  33. Sedlák E, Žoldák G, Antalík M, Sprinzl M. Thermodynamic properties of nucleotide-free EF-Tu from Thermus thermophilus in the presence of low-molecular weight effectors of its GTPase activity. Biochim Biophys Acta. 2002 May 20;1597(1):22–7.
×